In an illustrious career spanning over a decade, 36-year-old Sammy donned the West Indies jersey in 38 Tests, 126 ODIs and 68 T20Is.
Jason Roy's 57-ball 73 for the Gladiators went in vain.
The Gladiators have beaten the Zalmi in their last four meetings.
Quetta Gladiators won their campaign opener against Islamabad United by three wickets.
An impressive batting performance laid the foundation for Karachi Kings to clinch their first victory of the tournament.
Karachi Kings have a poor record against Peshawar Zalmi in PSL.
The Zalmi have beaten the Kings in their last four meetings with a head-to-head record of 8-2.
"I would like to thank the Peshawar Zalmi Fans & Team for this wondrous achievement," Javed Afridi said.
All the major stats and numbers recorded during the game between Peshawar Zalmi and Quetta Gladiators.
Both the Akmal brothers, Kamran and Umar, had good outings.